Cheslav Ivanovich Chenzinsky and Dmitry Leonidovich Romanowsky. have given rise to stain techniques of biological preparations with mixture of azur B, methylene blue and eosin Y.
The priority in polychrome staining of blood smears with mixture of azur B, methylene blue and eosin Y belongs to Romanowsky D.L..
Нis works initiated application and perfection of staining technique, which is used all over the world 120 years.
It is so called «Romanowsky stain» or «Romanowsky type stain».
Further a great number of doctors all over the world have taken part in perfection of a technique which is applied practically in each medical laboratory.
Works of Jenner, Leishman, May and Günvald, Nocht, Giemsa, Wright, Pappeheim and group of the authors who have developed ICSH reference technique of Romanowsky type staning, are most known.